Immunological applications of single-domain llama recombinant antibodies isolated from a naı̈ve library
نویسندگان
چکیده
Ana Monegal1, Diletta Ami1, Chiara Martinelli1,3, He Huang2, Marisa Aliprandi1, Paola Capasso1, Chiara Francavilla3, Giuseppe Ossolengo1 and Ario de Marco1,4 Consortium for Genomic Technologies, Biochemistry Unit, IFOM-IEO Campus, Via Adamello 16, 20139 Milano, Italy, Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R. China and SEMM, IFOM-IEO Campus, Via Adamello 16, 20139 Milano, Italy
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Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library.
We describe the rapid isolation of single-domain recombinant antibodies in VHH format from a pre-immune llama library created in our laboratory. Such naïve library has demonstrated to be a versatile tool and enabled the isolation of several different antibodies for any of the six proteins panned in parallel. The binders specific for human fibroblast growth factor receptor 1 (FGFR1) were success...
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